By A H Rebar; et al
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Extra info for A Guide to Hematology in Dogs and Cats
Canine eosinophils inconsistently separated from lymphocytes and monocytes. ® Does not distinguish between bands and segmented neutrophils. The degree of left shift cannot be determined from the QBC data. ® Tends to underestimate frequency of leukopenia. ® Algorithms used to calculate RBC and WBC counts assume normal cell size and structure. They are thus invalidated by conditions such as microcytosis, hypochromasia, and cell immaturity. Assessment of a stained blood smear is essential to rule out conditions that could lead to inaccurate differential counts.
Although recent breakthroughs in technology have reduced the cost so that these units are economically justified in most hospitals, they may not be economical in small practices or those that make minimal use of hematology. ® Examination of the blood film is still needed to detect abnormalities such as the presence of left shift, toxicity, reactive lymphocytes, blast cells, mast cells, microfilaria and red cell parasites. 26 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 27 3 LABORATORY METHODS IN HEMATOLOGY Figure 3-14 Laser flow cytometry detects and counts individual cells in microdroplets as they pass through a laser beam.
The smaller RBCs that lack central pallor are spherocytes. These cells are frequent in dogs with immune-mediated hemolytic disease. They can also occur in fragmentation hemolysis. A ghost RBC (arrow) is noted in the center of the field and suggests some degree of intravascular lysis (100x). Figure 4-26 Spherocytosis. If this were a feline blood smear, the RBC morphology would indicate regenerative anemia. The small RBC without central pallor would represent normal feline erythrocytes. However, this is canine blood and the small RBCs without central pallor are spherocytes.
A Guide to Hematology in Dogs and Cats by A H Rebar; et al